Written in English
Thesis (Ph. D.)--The Queen"s University of Belfast, 1974.
|The Physical Object|
Abstract. Paneth cells have been suggested to contribute to the elimination of excess metals into the intestinal lumen. The purpose of this study wat to investigate the changes of the zinc pools in rats subjected to functional loading with zinc salt by mean of both light and electron microscopical autometallography (AMG). It is believed that Paneth cells play an important role in the elimination and storage of heavy metals and the control of intestinal pathogens. Furthermore, patients with Acrodermatitis enteropathica, a genetic disorder of Zn absorption, show structural abnormalities in Paneth cells that disappear after Zn by: The metabolic role of zinc has been studied for many years, 1 but it was not until that Keilin and Mann offered the first concrete explanation of its mode of action. 2 They showed that the enzyme carbonic anhydrase contains % zinc as a part of its molecule and that zinc is essential to the elimination of carbon dioxide. Thus, zinc and iron have been shown to be components of two Cited by: Animal studies indicate that Paneth cell numbers, location, and granule morphology are altered by infection and zinc status. To date, the most compelling evidence in support of the role of Paneth cells in providing protection against enteric infection is from studies of mice transgenic to overproduce a human Paneth cell α-defensin, HD
Zinc transporter knockout studies in mice have provided direct evidence for a relationship between zinc and insulin metabolism in the pancreatic β-cell. Zinc uptake by Zip proteins (Zip6, Zip8, and Zip14) and subsequent sequestration into cytoplasmic secretory vesicles by ZnT proteins (ZnT3, ZnT5/ZnT7, and ZnT8) are critical for insulin. Loss of Paneth Cells Does Not Have Any Appreciable Effect on the Crypt-Villus Distribution of Components of the Microflora. Paneth cells are not present in adult FVB/N colonic crypts. Bacterial density increases along the duodenal-colonic axis of mice and man. The colonic crypts of many mammals, including mice, are colonized by bacteria. Zinc is a chemical element with the atomic num represented by the chemical symbol Zn.. Linus Pauling Institute. Zinc is an essential element in the nutrition of human beings, animals, and plants. Zinc is required in the genetic make-up of every cell and is an . Perhaps the most exciting area in contemporary zinc biology is the role of released zinc(II) ions in information transfer within cells and between cells. In pathways stimulus → increase of [Zn 2+] → targets, zinc(II) ions are signals. The preference of zinc(II) ions for specific coordination environments in proteins endows these signals.
The intestinal epithelium is the largest surface area that is exposed to various pathogens in the environment, however, in contrast to the colon the number of bacteria that colonize the small intestine is extremely low. Paneth cells, one of four major epithelial cell lineages in the small intestine, reside at the base of the crypts and have apically oriented secretory granules. The zinc-containing material secreted from Paneth cells, as observed in this study, may represent zinc aggregated with other Paneth cell secretions. This indicates that zinc may be important for the microbicidal activity of the cryptidins secreted by Paneth cells in addition to its role in cryptidin activation by matrilysin, a zinc-dependent. KEY WORDS: Paneth cell; Monoclonal antibody: Zinc-binding protein. Introduction Paneth cells are located deep within intestinal crypts and are mor- phologically well characterized, but their function has been not elucidated (). The high zinc content in Paneth cell granules has been demonstrated by formation of a purple-red deposit of zinc. Therefore, Paneth cells play a role in innate immunity by sensing bacteria and bacterial antigens. Paneth cells also store a large amount of zinc in their granules [35, 36]. Bacteria or bacterial components bind to PRRs, inducing the secretion of antibacterial molecules and zinc from Paneth cells .